Determination and analysis of protein interaction networks

Comunicaciones a congreso

The Micro Slit Gas Detector

We describe the first tests with a new proportional gas detector. Its geometry consists in slits opened in a copper metallized kapton foil with 30 micron anode strips suspended in these openings. In this way the multiplication process is similar to a standard MSGC. The fundamental difference is the absence of an insulating substrate around the anode. Also the material budget is significantly reduced, and the problems related to charging-up or polarization are removed. Ageing properties of this detector are under study.Comment: 13 pages tex file, 10 figures ep

Continuous-Wave Multiphoton Photoemission from Plasmonic Nanostars

Highly nonlinear optical processes, such as multiphoton photoemission, require high intensities, typically achieved with ultrashort laser pulses and, hence, were first observed with the advent of picosecond laser technology. An alternative approach for reaching the required field intensities is offered by localized optical resonances such as plasmons. Here, we demonstrate localized multiphoton photoemission from plasmonic nanostructures under continuous-wave illumination. We use synthesized plasmonic gold nanostars, which exhibit sharp tips with structural features smaller than 5 nm, leading to near-field-intensity enhancements exceeding 1000. This large enhancement facilitates 3-photon photoemission driven by a simple continuous-wave laser diode. We characterize the intensity and polarization dependencies of the photoemission yield from both individual nanostars and ensembles. Numerical simulations of the plasmonic enhancement, the near-field distributions, and the photoemission intensities are in good agreement with experiment. Our results open a new avenue for the design of nanoscale electron sources

Professionalization in environmental education facilities in Spain view from inside

[Resumo] A profesionalización é un dos temas que máis debates e reflexións despertou na última década no territorio español. O Libro Blanco da Educación Ambiental en España recomenda impulsar o recoñecemento profesional das e dos educadores ambientais. Desde os Seminarios Permanentes do CENEAM fixéronse distintas revisións e propostas, sempre desde a perspectiva temática do seminario. No de Equipamentos de Educación Ambiental acordouse realizar un estudo de ámbito estatal, no que se recollera o parecer tanto dos pro fesionais que estaban a traballar nos equipamentos, como dos responsables das distintas administracións. Neste artigo preséntanse os obxectivos, a metodoloxía e as conclusións de dito estudo.[Abstract] Professionalism is one of the topics that sparked debate and reflection on the past decade in the Spanish territory. The White Paper on Environmental Education in Spain recommended boost and professional recognition of environmental educators. From the Permanent Seminars CENEAM various revisions and proposals were drawn up, always from the perspective of the seminar topic. The Environmental Education facilities was agreed to conduct a study of state level, in which the views of both professionals who were working on the centre, as the heads of the various administrations pick. The objectives, methodology and conclusions estudy presented in this articl

Bilateral Pneumothoraces Following Central Venous Cannulation

We report the occurrence of a bilateral pneumothoraces after unilateral central venous catheterization of the right subclavian vein in a 70-year-old patient. The patient had no history of pulmonary or pleural disease and no history of cardiothoracic surgery. Two days earlier, she had a median laparotomy under general and epidural anaesthesia. Prior to the procedure, the patient was hemodynamically stable and her transcutaneous oxygen saturation was 97% in room air. We punctured the right pleural space before cannulation of the right subclavian vein. After the procedure, the patient slowly became hemodynamically instable with respiratory distress. A chest radiograph revealed a complete left-side pneumothorax and a mild right-side pneumothorax. The right-side pneumothorax became under tension after left chest tube insertion. The symptoms finally resolved after insertion of a right chest tube. After a diagnostic work-up, we suspect a congenital “Buffalo chests” explaining bilateral pneumothoraces and a secondary tension pneumothorax

Surface-Enhanced Raman Scattering Holography

Nanometric probes based on surface-enhanced Raman scattering (SERS) are promising candidates for all-optical environmental, biological and technological sensing applications with intrinsic quantitative molecular specificity. However, the effectiveness of SERS probes depends on a delicate trade-off between particle size, stability and brightness that has so far hindered their wide application in SERS imaging methodologies. In this Article, we introduce holographic Raman microscopy, which allows single-shot three-dimensional single-particle localization. We validate our approach by simultaneously performing Fourier transform Raman spectroscopy of individual SERS nanoparticles and Raman holography, using shearing interferometry to extract both the phase and the amplitude of wide-field Raman images and ultimately localize and track single SERS nanoparticles inside living cells in three dimensions. Our results represent a step towards multiplexed single-shot three-dimensional concentration mapping in many different scenarios, including live cell and tissue interrogation and complex anti-counterfeiting applications.Peer ReviewedPostprint (author's final draft

Protein co-evolution, co-adaptation and interactions

Co-evolution has an important function in the evolution of species and it is clearly manifested in certain scenarios such as host–parasite and predator–prey interactions, symbiosis and mutualism. The extrapolation of the concepts and methodologies developed for the study of species co-evolution at the molecular level has prompted the development of a variety of computational methods able to predict protein interactions through the characteristics of co-evolution. Particularly successful have been those methods that predict interactions at the genomic level based on the detection of pairs of protein families with similar evolutionary histories (similarity of phylogenetic trees: mirrortree). Future advances in this field will require a better understanding of the molecular basis of the co-evolution of protein families. Thus, it will be important to decipher the molecular mechanisms underlying the similarity observed in phylogenetic trees of interacting proteins, distinguishing direct specific molecular interactions from other general functional constraints. In particular, it will be important to separate the effects of physical interactions within protein complexes (‘co-adaptation') from other forces that, in a less specific way, can also create general patterns of co-evolution

Avoiding Interference in Planar Arrays through the Use of Artificial Neural Networks

[Abstract] This article implements an artificial neural network to find, through computer simulation, the excitations of a square planar array. The array is composed of 52 uniformly spaced subarrays, and has a quasi- in its radiation diagram. This simulation model includes the reduction of any signal interference in the shaped radiating zone after its position has been determined

Biometric parameters and biochemical composition of wild wreckfish (Polyprion americanus)

Wreckfish (Polyprion americanus) is considered to be a good candidate for European aquaculture. The main biometric parameters were recorded from 86 wild-caught fish (2.6-18 kg of total weight) for 14 months and the gonadosomatic index (GSI), hepatosomatic index (HIS) and viscerosomatic index (VSI) were obtained. Biochemical analyses of muscle, liver and gonads from wild wreckfish showed that wreckfish has a high level of protein in muscle, 84% of dry weight (DW), and low level of lipids (7% DW). Capture season, sex, size or weight of the fish did not have a strong effect on the variability observed in the levels of proteins, lipids and fatty acids of the different tissues. Reared wreckfish have more lipids in mature gonads than wild fish, which may be due to a more lipid-rich diet. However, some polyunsaturated fatty acids (PUFA) such as arachidonic acid (ARA) and docosahexaenoic acid (DHA) reached higher values in wild female gonads than in reared gonads, which could indicate the wreckfish has high requirements in these fatty acids. This study obtained valuable data on the biology and biochemical composition of wreckfish to estimate its nutritional requirements, which could be useful for the future of this species in aquaculture.European Union's Seventh Framework Programme Research, Technological development and demonstration (KBBE-2013-07)Postprint1,48